Thermo Fisher Scientific Phospho-Thioredoxin 1 (Thr100) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of TXN only when phosphorylated at Thr100.

Target Information

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor.
The reduced Trx functions as a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and acts as a potent general protein disulfide reductase with numerous roles in growth and redox regulation. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and transcription factors such as p53, NF-κB, and AP-1.
Trx also removes H₂O₂, particularly when coupled with methionine sulfoxide reductase or various peroxiredoxin isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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