Thermo Fisher Scientific Phospho-MYOD (Thr115) Polyclonal Antibody
인간, 생쥐, 랫트에서 인산화된 MyoD1(Thr115)을 검출하는 폴리클로날 항체. Western blot 및 ICC/IF에 적합. 고순도 액상 형태로 PBS/glycerol 버퍼에 보관. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MYOD1 (Accession P15172), corresponding to amino acid residues around phosphorylated Thr115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816782 |
Product Specific Information
Antibody detects endogenous levels of MyoD1 only when phosphorylated at Thr115.
Target Information
The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells to muscle cells. Forced expression of MyoD (human homolog is myf3) can activate muscle-specific genes or convert various cell types into muscle cells.
The regulatory domain of the MyoD gene product includes a 70 amino acid region with a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Other related genes sharing sequence homology for the basic HLH domain include myf5, myogenin (myf4), and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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