Thermo Fisher Scientific Desmosome/Hemidesmosome Chimeric Recombinant Rabbit Monoclonal Antibody (F12)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

F12

Immunogen

F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074519

Product Specific Information

Specificity: F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound. F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins-autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.

Target Information

Desmosomes and Hemidesmosomes are two types of intercellular junctions that play important roles in maintaining the structural integrity of tissues subjected to mechanical stress or shear forces. Desmosomes connect neighboring cells using cadherins and intermediate filaments, while Hemidesmosomes anchor epithelial cells to the underlying extracellular matrix using integrins. Both junctions are found in tissues like skin, heart, and muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.