Thermo Fisher Scientific FIBB Chimeric Recombinant Rabbit Monoclonal Antibody (8E5)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8E5

Immunogen

8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FIBB,uniProtId:P02675-1,ncbiNodeId:9606,antigenRange:45-51,antigenLength:491,antigenImageFileName:MA5-48078_FIBB_P02675-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48078_FIBB_P02675-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074521

Product Specific Information

Specificity: 8E5 binds to the amino-terminal region of the Beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.

Target Information

FIBB is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.