Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eBioscience
마우스 CD8 베타 분자를 인식하는 단클론 항체로, 세포독성 T세포의 면역표현 분석에 적합합니다. Flow cytometry, IHC(F), IP 등 다양한 응용에 사용 가능하며, 0.5 µg/test 권장. 높은 순도(>90%)와 낮은 응집도(<10%)를 보유한 액상 항체입니다.
- 카탈로그번호
- 14-0083-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 6 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 3 |
| Flow Cytometry (Flow) | 0.5 µg/test | 17 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Blocking Assay (BLOCK) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH35-17.2 (H35-17.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657757 |
Available Conjugated Formats
- Alexa Fluor 488
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Custom conjugation available upon request
Product Specific Information
Description:
The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on most thymocytes and peripheral cytotoxic T cells. CD8 binds to MHC class I and plays a crucial role in T cell development and activation.
Applications Reported:
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistochemical staining (frozen sections)
Applications Tested:
- Tested by flow cytometric analysis of mouse splenocytes and thymocytes
- Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize dilution empirically for best performance
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. Acting as a coreceptor with the T-cell receptor, CD8 recognizes antigens presented by class I MHC molecules. The functional coreceptor can be either a homodimer (two alpha chains) or a heterodimer (one alpha and one beta chain). Both chains share homology with immunoglobulin variable light chains. The CD8B gene encodes the beta chain isoforms, with multiple alternatively spliced variants described. A pseudogene is also located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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