
Thermo Fisher Scientific CD8b Monoclonal Antibody (eBio341 (341)), eBioscience
Rat CD8β 인식용 eBio341 (341) 단클론 항체로, Flow Cytometry, Western Blot, IHC, IP 등 다양한 분석에 사용 가능. MHC I 결합 및 T세포 신호전달 연구에 적합하며, 액상 형태로 4°C 보관. 고순도(>90%) 및 저응집(<10%) 보장.
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Thermo Fisher Scientific CD8b Monoclonal Antibody (eBio341 (341)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (IHC) | Assay-Dependent |
| Flow Cytometry (Flow) | 0.5 µg/test |
| Immunoprecipitation (IP) | Assay-Dependent |
| Functional Assay | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio341 (341) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657772 |
Additional Formats
Product Specific Information
The eBio341 monoclonal antibody reacts with rat CD8β, a member of the immunoglobulin superfamily that heterodimerizes with CD8α to form the CD8 antigen expressed on CD4+CD8+ thymocytes, MHC class I-restricted peripheral T cells, and some macrophages. CD8β expression depends on thymic T-cell development and participates in antigen presentation and signal transduction through Lck association.
Applications Reported: Suitable for flow cytometry, immunoprecipitation, immunoblotting (WB), and immunohistochemistry. Functional assays should use Functional Grade purified eBio341 (341) Product #16-0080.
Applications Tested: Validated by flow cytometric analysis of rat splenocytes and thymocytes. Recommended ≤0.5 µg per test (100 µL final volume). Optimal titration advised.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating efficient immune cell interactions. CD8 acts as a coreceptor with the T-cell receptor to recognize antigens presented by APCs via class I MHC molecules. The CD8 complex can form alpha-alpha homodimers or alpha-beta heterodimers, both homologous to immunoglobulin variable light chains. This gene encodes CD8β isoforms and has multiple alternatively spliced variants, including a pseudogene on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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