Thermo Fisher Scientific mTOR Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human-derived tissues and cells.
BLAST analysis suggests cross-reactivity with mTOR protein from rat and mouse (100% homology with the immunizing sequence).
Partial reactivity expected against mTOR homologues from zebrafish (94%) and dog (89%).
Reactivity against other sources is not known.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation.
Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with various cancers.
FRAP1 is the target of rapamycin and its analogues, used as immunosuppressants and cancer therapeutics.
Mutations in this gene are linked to Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.