Thermo Fisher Scientific mTOR Polyclonal Antibody
mTOR 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, ELISA, IP에 적합. Human, Mouse, Rat에 반응하며 phosphorylated 및 non-phosphorylated 형태 모두 인식. Affinity purified, 0.9 mg/mL 농도의 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:50,000–1:100,000 |
| Immunoprecipitation (IP) | 1:250–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 2430–2460 of human mTOR. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human-derived tissues and cells.
BLAST analysis suggests cross-reactivity with mTOR protein from rat and mouse (100% homology with the immunizing sequence).
Partial reactivity expected against mTOR homologues from zebrafish (94%) and dog (89%).
Reactivity against other sources is not known.
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation.
Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with various cancers.
FRAP1 is the target of rapamycin and its analogues, used as immunosuppressants and cancer therapeutics.
Mutations in this gene are linked to Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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