Thermo Fisher Scientific Phospho-MARK2 (Thr595) Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1, and T568 on MARK4. A BLAST analysis suggests reactivity with human, dog, bovine, mouse, and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with MARK2 homologues from other species has not been determined.

Target Information

MARK2 is a serine/threonine protein kinase involved in the control of cell polarity and microtubule stability. Several cDNA clones encoding two isoforms of the human ser/thr protein kinase EMK1 have been identified. These isoforms differ by a 162-bp alternative exon, resulting in two forms — one containing the exon and the other lacking it. Both forms are coexpressed in multiple cell lines and tissues. The human EMK1 gene is encoded by a single mRNA ubiquitously expressed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.