Thermo Fisher Scientific Phospho-MARK2 (Thr595) Polyclonal Antibody
Phospho-MARK2 (Thr595) 특이성을 가진 Rabbit Polyclonal Antibody로, 인산화된 MARK2 단백질 검출에 최적화됨. WB, ICC/IF, ELISA에 사용 가능하며 다양한 종에 반응. 친화성 크로마토그래피로 정제된 액상 항체로 -20°C에서 안정적으로 보관.
- 카탈로그번호
- 600-401-895
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-MARK2 (Thr595) Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:1,000–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to amino acids surrounding Thr595 of Human MARK2 isoform a. The peptide was phosphorylated at T595. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1, and T568 on MARK4. A BLAST analysis suggests reactivity with human, dog, bovine, mouse, and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with MARK2 homologues from other species has not been determined.
Target Information
MARK2 is a serine/threonine protein kinase involved in the control of cell polarity and microtubule stability. Several cDNA clones encoding two isoforms of the human ser/thr protein kinase EMK1 have been identified. These isoforms differ by a 162-bp alternative exon, resulting in two forms — one containing the exon and the other lacking it. Both forms are coexpressed in multiple cell lines and tissues. The human EMK1 gene is encoded by a single mRNA ubiquitously expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.