Thermo Fisher Scientific Collagen II Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

ELISA (ELISA)

1:10,000-1:50,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type II from adult human knee cartilage and bovine nasal cartilage if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen II,uniProtId:P02458-2,ncbiNodeId:9606,antigenRange:1-1487,antigenLength:1487,antigenImageFileName:PA1-28531_Collagen_II_P02458-2_Rabbit.svg,antigenImageFileNamePDP:PA1-28531_Collagen_II_P02458-2_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Collagen II,uniProtId:P02459-1,ncbiNodeId:9913,antigenRange:1-1487,antigenLength:1487,antigenImageFileName:PA1-28531_Collagen_II_P02459-1_Rabbit.svg,antigenImageFileNamePDP:PA1-28531_Collagen_II_P02459-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1956961

Product Specific Information

The reactivity of the antibody is greatly diminished under denaturing and reducing conditions.

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.