Thermo Fisher Scientific Phospho-RIP3 (Thr231, Ser232) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within mouse RIP3 aa 201-250/518. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RIP3,uniProtId:Q9QZL0-1,ncbiNodeId:10090,antigenRange:201-250,antigenLength:486,antigenImageFileName:PA5-144629_RIP3_Q9QZL0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144629_RIP3_Q9QZL0-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3092357

Product Specific Information

Positive control: L929 cell lysate treated with 20 µM Z-VADthen treated with 20 ng/mL mTNF-alpha for 2 hours, L929 cell lysate treated with 20 µM Z-VAD then treated with 20 ng/mL mTNF-alpha for 3.5 hours.

Predicted band size: 53 kDa

Subcellular Location: Cytosol, Nucleus.

Target Information

RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.