Thermo Fisher Scientific Leptin Receptor (LEPR)/Ob-R/CD295 Monoclonal Antibody (LEPR, 4301)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature (RT).
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.

Target Information

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily, playing a critical role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of OB-R by leptin triggers downstream signaling through the JAK/STAT pathway. Several isoforms of OB-R with varying signaling capabilities exist. The long form of OB-R is expressed in the choroid plexus and hypothalamus, areas involved in satiety and energy expenditure control. Mutations in OB-R (db) or leptin (ob) genes in mice cause early-onset obesity and metabolic abnormalities such as hyperinsulinemia, insulin resistance, hyperglycemia, and increased adipose tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.