Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 436, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers.

• CD8αβ: majority of thymocytes and αβ TCR T cells
• CD8αα: γδ TCR T cells, intestinal IELs, dendritic cells

CD8 binds MHC class I and associates with protein tyrosine kinase p56^lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Super Bright 436 is excited at 405 nm and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules.

• Expressed on T cells, NK cells, monocytes, and dendritic cells
• Exists as CD8αβ heterodimer or CD8αα homodimer
• Involved in signal transduction, T cell differentiation, and activation

Upon MHC-I/peptide ligation, CD8 recruits Lck kinase, leading to lymphokine production, motility, and CTL activation.
Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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