Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 436, eBioscience
마우스 CD8a 항원을 인식하는 53-6.7 클론의 Rat IgG2a 단클론 항체로, Super Bright 436 형광체가 결합되어 있음. Flow Cytometry에 최적화되어 있으며 0.25 µg/test로 사용. Violet laser(405 nm)에서 여기되어 436 nm에서 방출. T세포 활성화 및 분화 연구용으로 적합.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 87 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716980 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers.
- CD8αβ: majority of thymocytes and αβ TCR T cells
- CD8αα: γδ TCR T cells, intestinal IELs, dendritic cells
CD8 binds MHC class I and associates with protein tyrosine kinase p56^lck, playing a role in T cell development and activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.
Super Bright 436 is excited at 405 nm and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules.
- Expressed on T cells, NK cells, monocytes, and dendritic cells
- Exists as CD8αβ heterodimer or CD8αα homodimer
- Involved in signal transduction, T cell differentiation, and activation
Upon MHC-I/peptide ligation, CD8 recruits Lck kinase, leading to lymphokine production, motility, and CTL activation.
Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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