Thermo Fisher Scientific Caspase 9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_0012202) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caspase 9,uniProtId:P55211-1,ncbiNodeId:9606,antigenRange:139-416,antigenLength:416,antigenImageFileName:PA5-116498_Caspase_9_P55211-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116498_Caspase_9_P55211-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901129

Product Specific Information

Positive Samples: HeLa, Jurkat, Mouse brain, Rat brain

Immunogen sequence: VGALESLRGN ADLAYILSME PCGHCLIINN VNFCRESGLR TRTGSNIDCE KLRRRFSSLH FMVEVKGDLT AKKMVLALLE LAQQDHGALD CCVVVILSHG CQASHLQFPG AVYGTDGCPV SVEKIVNIFN GTSCPSLGGK PKLFFIQACG GEQKDHGFEV ASTSPEDESP GSNPEPDATP FQEGLRTFDQ LDAISSLPTP SDIFVSYSTF PGFVSWRDPK SGSWYVETLD DIFEQWAHSE DLQSLLLRVA NAVSVKGIYK QMPGCFNFLR KKLFFKTS

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer`s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.