Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), PE, eBioscience
CD266 (TWEAK Receptor) 검출용 ITEM-4 단클론 항체로, 인간 및 생쥐 시료에 반응합니다. PE 형광 표지로 유세포분석에 최적화되어 있으며, Fn14 관련 세포 이동·증식 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), PE, eBioscience™
Applications and Tested Dilution
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ITEM-4 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668007 |
Available Formats
Product Specific Information
The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but lacks a death domain (DD) in the cytoplasmic region.
Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody (CARL-1). Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. ITEM-4 cross-reacts with mouse Fn14, which is constitutively expressed by the Colon 26 cell line.
The ITEM-4 antibody has demonstrated blocking functionality.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on human or mouse Fn14-transfected cells.
Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TWEAK is a member of the TNF family, initially described as a weak inducer of apoptosis. Its receptor, TweakR (Fn14), was identified from a HUVEC cDNA library and belongs to the TNF receptor superfamily. TweakR initiates proliferative signaling by binding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver and may play a role in vascular cell migration and angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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