Thermo Fisher Scientific ERK2 Recombinant Rabbit Monoclonal Antibody (SZ25-01)
ERK2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등에 사용 가능. 높은 특이성과 재현성을 제공하며, HEK293 세포 발현 시스템으로 생산됨. 단백질 A로 정제되어 안정적이며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ25-01 |
| Immunogen | Synthetic peptide within Human ERK2 (aa 311–360) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809319 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that functions as an activator of p90 RSK, MSK, ELK1, and Stat3, and plays a crucial role in the MAPK signaling pathway.
ERKs serve as integration points for multiple biochemical signals and regulate various cellular processes including:
- Proliferation
- Differentiation
- Transcription regulation
- Development
Activation requires phosphorylation by upstream kinases, after which ERK2 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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