Thermo Fisher Scientific H3R2me1 Recombinant Rabbit Monoclonal Antibody (ARC0124)
H3R2me1 단일메틸화 히스톤 H3 인식용 재조합 토끼 단클론 항체로, WB, ELISA, ChIP 등 다양한 응용에 적합. HEK293 발현 시스템 기반 IgG 형 항체이며, 고순도 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| ChIP assay | 1:20–1:100 |
| Dot blot (DB) | 1:500–1:1,000 |
| CUT&RUN (C&R) | 10⁵ cells / 1 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0124 |
| Immunogen | Synthetic monomethylated peptide around R2 of human Histone H3 (Q16695) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849231 |
Product Specific Information
Immunogen sequence:
MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified to change chromatin structure or allow specific transcription factor binding. The N-terminal tail of histone H3 undergoes various modifications such as methylation, acetylation, and phosphorylation, influencing key cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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