Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (CRA1 (AER37))

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1-5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Shrew

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

CRA1 (AER37)

Immunogen

Region of FceR1 alpha (aa 26-197).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1957996

Product Specific Information

MA1-4997 detects Fc epsilonR1 alpha from human samples. The CRA1 (AER37) monoclonal antibody reacts with the Fc epsilonR1 alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor bindings.

MA1-4997 has been successfully used in Western blot, immunocytochemistry, flow cytometry, and immunohistochemistry (paraffin, frozen) applications.

The MA1-4997 immunogen is region of Fc epsilonR1 alpha which does not overlap with the IgE binding site.

The epitope has been mapped to amino acids 26-110.

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.