Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (13/45/31-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1-4 µg/mL

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Immunocytochemistry (ICC/IF)

1-4 µg/mL

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Flow Cytometry (Flow)

5-10 µL/1x10^6 cells

-

ELISA (ELISA)

0.1-0.4 µg/mL

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Immunoprecipitation (IP)

4 µg/mL

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Product Specifications

Published species

Tag

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

13/45/31-2

Immunogen

Recombinant (His) 6-p53 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_612244

Product Specific Information

MA1-4806 detects any kind of 6X-His tagged proteins in cells and complex cellular lysates.

MA1-4806 has been successfully used in flow cytometry, immunohistochemistry (frozen), immunofluorescence, Western blot, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody.

The MA1-4806 immunogen is recombinant (His) 6-p53 protein

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.