Thermo Fisher Scientific PMCA ATPase Monoclonal Antibody (5F10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 66 publications 66 publications

Immunohistochemistry (IHC)

-

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Amphibian, Dog, Chicken, Cat, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

Published species

Amphibian, Bovine, Cat, Dog, Hamster, Human, Insect, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5F10

Immunogen

Purified human erythrocyte calcium ATPase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PMCA ATPase,uniProtId:P20020-3,ncbiNodeId:9606,antigenRange:1-1220,antigenLength:1220,antigenImageFileName:MA3-914_PMCA_ATPase_P20020-3_House_mouse.svg,antigenImageFileNamePDP:MA3-914_PMCA_ATPase_P20020-3_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061566

Product Specific Information

MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.

MA3-914 has been successfully used in Western blot, FACS, immunohistochemistry, immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~140 kDa protein representing PMCA ATPase and bands at 95kDa and 180 kDa which probably represent products of aggregation and/or natural proteolytic products of the pump from rat liver membrane preparations. Immunohistochemical staining of PMCA ATPase with MA3-914 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

MA3-914 can be used on both frozen and formalin-fixed paraffin-embedded tissues.

The MA3-914 antigen is purified human erythrocyte calcium ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.

Target Information

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.