Thermo Fisher Scientific RC3H1 Polyclonal Antibody
RC3H1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot 및 IHC에 적합하며, 인간, 마우스, 랫트 시료에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide near the carboxy terminus of human RC3H1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at –20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551871 |
Product Specific Information
- A suggested positive control is HeLa cell lysate.
- PA5-34519 can be used with blocking peptide PEP-1562.
Target Information
The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING-type ubiquitin ligase protein family. Mutation M199R leads to excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, which exhibit high IL-21 levels and autoantibody titers. Complete loss of RC3H1 causes early death and immune deregulation but not autoimmunity, suggesting that the mutant form is more disruptive to immune function than its absence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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