Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioABL-93 (ABL-93)), Alexa Fluor 488, eBioscience
마우스 CD107b(LAMP-2) 인식 단일클론 항체로, Alexa Fluor 488로 표지되어 있습니다. 세포 내 염색 및 유세포분석(Flow Cytometry)에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 4°C에서 암소 보관하며, 동결 금지.
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Applications
Immunohistochemistry (IHC)
- Tested dilution: Not specified
- Publications: View 1 publication
Flow Cytometry (Flow)
- Tested dilution: 1 µg/test
- Publications: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioABL-93 (ABL-93) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_657566 |
Additional Formats
Product Specific Information
Description:
The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated protein (~120 kDa) expressed in lysosomal membranes and transiently on the surface of cytolytic T cells during degranulation. It has been reported that CD107b may be identical to the mouse Mac-3 antigen.
Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis, and for surface staining in flow cytometric degranulation assays.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of thioglycollate-elicited peritoneal macrophages. Recommended ≤1 µg/test (in 100 µL final volume). Optimal titration is advised for each assay.
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b)
LAMP2 is a member of a family of lysosomal membrane glycoproteins involved in lysosome biogenesis, fusion with phagosomes, and autophagy regulation. It provides selectins with carbohydrate ligands and may play a role in tumor metastasis and lysosomal maintenance.
The mature LAMP2 protein (~100–130 kDa) is heavily glycosylated and consists of:
- A large amino-terminal intra-lysosomal domain
- A hydrophobic transmembrane domain
- A short carboxyl-terminal cytoplasmic tail
LAMP2 deficiency leads to accumulation of autophagosomes and is associated with Danon’s disease.
Variants include LAMP-2A, -2B, and -2C.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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