Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioABL-93 (ABL-93)), Alexa Fluor 488, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested dilution: 1 µg/test
• Publications: View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• eFluor 660
• FITC
• Request custom conjugation

Product Specific Information

Description:
The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated protein (~120 kDa) expressed in lysosomal membranes and transiently on the surface of cytolytic T cells during degranulation. It has been reported that CD107b may be identical to the mouse Mac-3 antigen.

Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis, and for surface staining in flow cytometric degranulation assays.

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of thioglycollate-elicited peritoneal macrophages. Recommended ≤1 µg/test (in 100 µL final volume). Optimal titration is advised for each assay.

• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b)
LAMP2 is a member of a family of lysosomal membrane glycoproteins involved in lysosome biogenesis, fusion with phagosomes, and autophagy regulation. It provides selectins with carbohydrate ligands and may play a role in tumor metastasis and lysosomal maintenance.
The mature LAMP2 protein (~100–130 kDa) is heavily glycosylated and consists of:

• A large amino-terminal intra-lysosomal domain
• A hydrophobic transmembrane domain
• A short carboxyl-terminal cytoplasmic tail

LAMP2 deficiency leads to accumulation of autophagosomes and is associated with Danon’s disease.
Variants include LAMP-2A, -2B, and -2C.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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