Thermo Fisher Scientific SKG-1 Polyclonal Antibody
SGK-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. Protein A로 정제된 액상 형태이며, 5 mg/mL 농도. 인체 및 설치류 등 다양한 종에 반응하며, 연구용으로 적합.
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Thermo Fisher Scientific SKG-1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10–20 µg/mL |
| ELISA | 1:4,000–1:16,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a C-terminal region near amino acids 400–431 of human SGK-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-SKG-1 Antibody is directed against human SGK-1 protein.
BLAST analysis suggests reactivity with human, macaque, and chimpanzee SGK-1 based on 100% homology for the immunogen sequence.
Expected cross-reactivity with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit, and spiny dogfish (92% sequence homology).
Cross-reactivity with other species has not been determined.
Target Information
SGK (SGK1) is a serine/threonine kinase that may be a transcriptional target for p38/MAPK in hepatoma cells.
It plays an important role in cellular stress response and survival.
SGK1 is transcriptionally activated by serum, glucocorticoids, osmotic stress, and hormones.
It phosphorylates and negatively regulates pro-apoptotic FOXO3A.
SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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