Thermo Fisher Scientific Jagged 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Target Information

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.