Thermo Fisher Scientific Jagged 1 Polyclonal Antibody
Jagged 1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Human 및 Mouse 반응성, Protein A 정제, 1 mg/mL 농도. 연구용으로 Alagille syndrome 관련 Jagged 1 신호 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids near the N-terminus of human Jagged-1 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with Jagged-1 protein from human, chimpanzee, rat, and mouse (100% homology with immunizing sequence). Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on sequence homologies. Reactivity against other homologues is not known.
Target Information
The Jagged 1 protein encoded by JAG1 is the human homolog of the Drosophila jagged protein. Human Jagged 1 acts as a ligand for the receptor Notch 1, a homolog of the Drosophila notch receptor. Mutations in Jagged 1 cause Alagille syndrome. Jagged 1 signaling through Notch 1 also plays a role in hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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