Thermo Fisher Scientific Coilin Monoclonal Antibody (1H10)
Western blot, ICC/IF, IP에 적합한 Coilin 단클론 항체로, HeLa 세포 용해물이 양성 대조로 추천됨. 마우스 IgG2b 항체이며, 비결합형 액상 형태로 1 mg/mL 농도 제공. 연구용으로 Cajal body 관련 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Immunoprecipitation (IP) | 2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H10 |
| Immunogen | Recombinant human protein purified from E. coli (His-Coilin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2538728 |
Product Specific Information
A suggested positive control for this product is HeLa cell lysate.
Target Information
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles involved in post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization, while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely affect its localization and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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