Thermo Fisher Scientific PPAR gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,500

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 3 publications 3 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(284) M G E D K I K F K H I T P L(298) of mouse PPAR gamma 2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PPAR gamma,uniProtId:P37238-1,ncbiNodeId:10090,antigenRange:284-298,antigenLength:505,antigenImageFileName:PA3-821A_PPAR_gamma_P37238-1_Rabbit.svg,antigenImageFileNamePDP:PA3-821A_PPAR_gamma_P37238-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2166056

Product Specific Information

PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits.

PA3-821A has been successfully used in IF and Western blot procedures. Previous batches of this antibody have been used in gel shift, immunocytochemistry, and immunofluoresence procedures. By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches gives predominant nuclear staining.

The PA3-821A immunizing peptide corresponds to amino acid residues 284-298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1, but exhibits no significant homology with PPAR alpha or NUC1. This sequence is completely conserved in mouse, human, and rat.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.