Thermo Fisher Scientific PPAR gamma Polyclonal Antibody
PPAR gamma 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IF 등 다양한 응용에 사용 가능하며, 핵 내 PPARγ 검출에 적합합니다. -20°C 보관, sodium azide 함유.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,500 | View 16 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:250 | View 3 publications |
| Flow Cytometry (Flow) | – | View 3 publications |
| Immunoprecipitation (IP) | – | View 1 publication |
| ChIP assay (ChIP) | – | View 3 publications |
| Gel Shift (GS) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues M(284)-L(298) of mouse PPAR gamma 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2166056 |
Product Specific Information
PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits.
PA3-821A has been successfully used in immunofluorescence (IF) and Western blot (WB) applications. Previous batches have also been used in gel shift, immunocytochemistry, and immunofluorescence assays.
By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches shows predominant nuclear staining.
The immunizing peptide corresponds to amino acid residues 284–298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1 and across mouse, human, and rat species, but shows no significant homology with PPAR alpha or NUC1.
Target Information
The PPAR gamma gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and regulate transcription of various genes.
Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma.
PPAR-gamma regulates adipocyte differentiation and is implicated in diseases such as obesity, diabetes, atherosclerosis, and cancer.
Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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