Thermo Fisher Scientific IGF2R Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Dot blot (DB)

1:1,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

300 kDa cation-independent receptor purified from adult bovine liver tissue. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IGF2R,uniProtId:P08169-1,ncbiNodeId:9913,antigenRange:1-2499,antigenLength:2499,antigenImageFileName:PA3-850_IGF2R_P08169-1_Rabbit.svg,antigenImageFileNamePDP:PA3-850_IGF2R_P08169-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2138964

Product Specific Information

PA3-850 detects cation-independent mannose-6-receptor(CI-MPR) in human, bovine, mouse and non-human primate cells.

PA3-850 has been successfully used in ICC/IF, Dot blot and Western blotting procedures. By Western blot, it detects a 300 kDa protein representing CI-MPR under non-reducing conditions.

The PA3-850 immunogen is a 300 kDa cation-independent receptor purified from adult Bovine liver tissue.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR`s is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.