Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 661
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a transcription factor essential for thymic development and Th2 differentiation. Gata-3 expression occurs in the CNS, skin, mammary glands, and kidney during embryogenesis. It is critical for T cell commitment and survival, predominantly expressed in CD4 single-positive thymocytes.

• Recognizes both 50 and 45 kDa Gata-3 isoforms.
• Requires Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) for intracellular staining.
• Crossreactivity observed in rhesus monkeys.
• Reported for intracellular staining followed by flow cytometric analysis.
• Tested at 5 µL (0.5 µg) per test with the Foxp3/Transcription Factor Staining Buffer Set.
• A test is defined as staining a 100 µL cell sample (10⁵–10⁸ cells).

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Handling Notes:

• Light sensitive – protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet.

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

GATA3 is a zinc finger transcription factor regulating T-cell receptor (TCR) genes (alpha, beta, gamma, delta). It binds enhancer elements of all 4 TCR genes, promoting T-cell lineage expression. GATA3 mRNA is abundant in T cells, absent in B cells and macrophages. The human gene spans 6 exons over 17 kb, encoding two conserved zinc fingers similar to GATA1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지