Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 661
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells.
SK7 and UCHT1 clones cross-block binding, suggesting overlapping epitopes, whereas OKT3 and SK7 recognize different epitopes.
The antibody SK7 recognizes chimpanzee CD3.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use at 5 µL (0.5 µg)/test.
A test corresponds to staining a cell sample in 100 µL volume; cell number range: 10⁵–10⁸ cells/test.

Brilliant Ultra Violet™ 615 (BUV615):
A tandem dye emitting at 615 nm for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T-cell cytoplasm and regulates TCR surface expression.
CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on human chromosome 11.
CD3 plays roles in TCR-induced growth arrest, survival, and proliferation.
Expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells; not expressed on B or NK cells.
Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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