Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.03 µg)/test
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Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 661, 737, 805
• Brilliant Violet 480
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, 725
• NovaFluor Yellow 660, 730
• PE, PE-Cyanine5, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (FcγRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa.
CD16 includes two genes:

• CD16A (FcγRIIIA): Expressed on macrophages and NK cells (transmembrane form)
• CD16B (FcγRIIIB): Expressed on neutrophils (GPI-anchored form)

Polymorphisms (NA-1, NA-2) in CD16B affect phagocytic capacity. CD16 binds IgG immune complexes, showing preference for IgG1 and IgG3. Upon binding, it triggers signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells.
Use at 5 µL (0.03 µg) per test in a final volume of 100 µL.
Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity: Protect from light (photo-oxidation sensitive).
Fixation: Samples can be stored in:

• IC Fixation Buffer (Product #00-822-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD16 (FcγRIII) encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance and antibody-dependent cellular cytotoxicity (ADCC).

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (transmembrane glycoprotein), neutrophils (PI-linked form via FCGR3B)
• Clinical relevance: Mutations linked to Immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
• Variants: Multiple alternatively spliced isoforms exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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