Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), PE-Cyanine7, eBioscience
인간 CD19 항원을 인식하는 HIB19 클론 단일클론 항체로, PE-Cyanine7 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test 농도로 사용 가능합니다. 빛에 민감하므로 암조건에서 보관하며, 4°C에서 냉암소 보관을 권장합니다.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), PE-Cyanine7, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582279 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Yellow series
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780
- Custom conjugation available upon request
Product Specific Information
Description:
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to mediate signaling, tyrosine phosphorylation, calcium flux, and B cell proliferation. The HIB19 and SJ25C1 antibodies recognize overlapping epitopes.
Applications Reported:
- Flow cytometric analysis.
Applications Tested:
- Pre-titrated and validated for flow cytometry using normal human peripheral blood cells.
- Recommended use: 5 µL (0.25 µg) per test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
Light Sensitivity:
- Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation:
- Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
- Clone-specific performance should be tested empirically.
Excitation/Emission:
- Excitation: 488–561 nm
- Emission: 775 nm
- Laser: Blue, Green, Yellow-Green
Filtration:
- 0.2 µm post-manufacturing filtered.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 serves as a functional regulator of B cell proliferation and differentiation and is expressed in all B cell precursor leukemias. It lowers the threshold for BCR-dependent stimulation and acts as a signal-amplifying co-receptor. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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