Thermo Fisher Scientific Streptavidin Polyclonal Antibody, FITC
FITC 결합 Streptavidin 폴리클로날 항체로 Western blot, ICC/IF, ELISA에 사용 가능. Rabbit IgG 기반으로 높은 특이성과 안정성 제공. Streptomyces avidinii 유래 Streptavidin 항원에 반응하며 Biotin 결합 분석에 적합. 연구용으로만 사용.
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- 200-402-095x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific Streptavidin Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Streptavidin (Streptomyces avidinii) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Streptavidin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, and Streptavidin. No reaction was observed against Avidin.
Target Information
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds tightly to vitamin biotin with a Kd of ~10⁻¹⁴ mol/L.
The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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