Thermo Fisher Scientific H3K4me1 Polyclonal Antibody
Human Histone H3K4me1에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP, ChIP 등 다양한 응용 가능. Protein A로 정제된 고순도 항체이며, 액상 형태로 -20°C 보관. 인간 및 다종 교차 반응 예측.
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Thermo Fisher Scientific H3K4me1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 2–5 µg/mL |
| ELISA | 1:3,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2. A BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. It is predicted to react with many species including Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or enable the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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