Thermo Fisher Scientific PARP1 (internal) Polyclonal Antibody
인간 PARP1 단백질에 특이적인 Rabbit Polyclonal Antibody로, Western blot에 1:1,000 희석 비율로 사용 가능. Protein A로 정제된 액상 형태이며, 1 mg/mL 농도. -20°C에서 보관하며 반복적인 동결-해동을 피해야 함.
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Thermo Fisher Scientific PARP1 (internal) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with the C-terminus region of the human PARP1 autocatalytic domain recombinant protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human PARP1 protein. No cross-reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form, NAD) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: 200-401-X51_PARP1_P09874-1_Rabbit.svg)
(이미지: 200-401-X51_PARP1_P09874-1_Rabbit_PDP.jpeg)
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