Thermo Fisher Scientific Protein G Polyclonal Antibody, FITC
FITC로 표지된 Protein G 폴리클로날 항체로, Western blot, ICC/IF, ELISA, IP 등 다양한 면역분석에 사용 가능. Streptococcus 유래 Protein G를 면역원으로 하며, 토끼 IgG로 제작. 498/517 nm의 형광 특성. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein G (Streptococcus species) |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, and Protein G.
Target Information
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcus bacteria, similar to Protein A but with differing binding specificities.
It is a cell surface protein useful for purifying antibodies through binding to the Fab and Fc regions. The native molecule also binds albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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