Thermo Fisher Scientific MyoD1 Recombinant Rabbit Monoclonal Antibody (MYOD1, 3418R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells to muscle cells. Forced expression of the MyoD (human homolog is myf3) gene in various normal and neoplastic cells can either convert the cells to muscle cells or activate otherwise transcriptionally inactive muscle-specific genes.
The regulatory domain of the MyoD gene product lies within a 70 amino acid region containing a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Subsequent studies identified three other genes sharing sequence homology for the basic HLH domain of MyoD: myf5, myogenin (myf4), and myf6 (MRF4, herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.