Thermo Fisher Scientific MyoD1 Recombinant Rabbit Monoclonal Antibody (MYOD1, 3418R)
MyoD1 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간 시료에 반응합니다. IHC(P)에서 1–2 µg/mL로 사용 가능하며, 포르말린 고정 조직 염색에 적합합니다. Protein A로 정제된 액상 항체로, 보존제가 없으며 -20°C 이하에서 보관합니다.
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- 4654-RBM8-P1ABX
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MYOD1, 3418R |
| Immunogen | Recombinant fragment (around aa1–100) of human MyoD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The MyoD (Myogenic determination gene) was first identified for its ability to convert embryonic mouse fibroblast cells into muscle cells.
Forced expression of MyoD (human homolog myf3) in various normal and neoplastic cells can induce muscle cell conversion or activate otherwise silent muscle-specific genes.
The regulatory domain of MyoD lies within a 70 amino acid region containing a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Related genes sharing sequence homology in the basic HLH domain include myf5, myogenin (myf4), and myf6 (MRF4, herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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