Thermo Fisher Scientific Ankyrin B Monoclonal Antibody (N105/13), PerCP
Ankyrin-B 단백질을 검출하기 위한 PerCP 결합 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 생쥐, 랫트 반응성 보유. 200kDa 단백질 검출에 특이적이며 Ankyrin-G와 교차 반응 없음. 단백질 G 정제, 4°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N105/13 |
| Immunogen | Synthetic peptide amino acids 203–496 of human Ankyrin-B |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931982 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45528 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 200 kDa. No cross-reactivity against Ankyrin-G.
This antibody was formerly sold as clone S105-13.
Target Information
This gene encodes a member of the ankyrin family of proteins that link integral membrane proteins to the spectrin-actin cytoskeleton. Ankyrins play key roles in cell motility, activation, proliferation, contact, and maintenance of specialized membrane domains.
Most ankyrins consist of three structural domains:
- Amino-terminal domain containing multiple ankyrin repeats
- Central region with a conserved spectrin binding domain
- Carboxy-terminal regulatory domain, which is variable
The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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