Thermo Fisher Scientific Ankyrin B Monoclonal Antibody (N105/13), APC
Ankyrin-B 단백질 검출용 마우스 단클론 항체로 APC 형광표지. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 반응성. 고순도 Protein G 정제, 4°C 보관, 보존제 없음. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N105/13 |
| Immunogen | Synthetic peptide amino acids 203–496 of human Ankyrin-B |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931980 |
Additional Formats
- FITC (Cat. MA5-45527)
- PerCP (Cat. MA5-45528)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45526 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 200 kDa. No cross-reactivity against Ankyrin-G.
This antibody was formerly sold as clone S105-13.
Target Information
This gene encodes a member of the ankyrin family of proteins that link integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in cell motility, activation, proliferation, contact, and maintenance of specialized membrane domains.
Most ankyrins consist of:
- Amino-terminal domain with multiple ankyrin repeats
- Central region with conserved spectrin binding domain
- Carboxy-terminal regulatory domain (least conserved, variable)
The encoded protein is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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