Thermo Fisher Scientific Ankyrin B Monoclonal Antibody (N105/13), FITC
Ankyrin-B 단백질 검출용 FITC 결합 단클론 항체. WB, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 시료 반응. 고순도 Protein G 정제, 4°C 암소 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Ankyrin B Monoclonal Antibody (N105/13), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N105/13 |
| Immunogen | Synthetic peptide amino acids 203–496 of human Ankyrin-B |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931981 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45527 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 200 kDa. No cross-reactivity against Ankyrin-G.
This antibody was formerly sold as clone S105-13.
Target Information
This gene encodes a member of the ankyrin family of proteins that link integral membrane proteins to the underlying spectrin–actin cytoskeleton. Ankyrins play key roles in cell motility, activation, proliferation, contact, and maintenance of specialized membrane domains.
Most ankyrins are composed of three structural domains:
- Amino-terminal domain with multiple ankyrin repeats
- Central region with conserved spectrin binding domain
- Carboxy-terminal regulatory domain (least conserved and variable)
The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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