Thermo Fisher Scientific TCR V alpha 24 J alpha 18 Monoclonal Antibody (6B11), eBioscience

Thermo Fisher Scientific TCR V alpha 24 J alpha 18 Monoclonal Antibody (6B11), eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
Publications: View 5 publications

Product Specifications

Available Conjugated Formats

• APC
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 6B11 monoclonal antibody recognizes an epitope within the CDR3 region formed by the germ-line configuration of Vα24 and Jα18 of the TCR α locus, resulting in expression of an invariant TCR characteristic of invariant NK T (iNKT) cells.
This human TCR is homologous to murine Vα14Jα18 and pairs primarily with Vβ11 chains. iNKT cells are CD1d-restricted and activated by CD1d-presented glycolipid ligands, leading to rapid production of IL-4, IL-13, and IFN-γ.
Although they exhibit NK-like cytolytic activity, iNKT cells serve as immune regulators by producing large amounts of Th1 and Th2 cytokines in autoimmune, infectious, and cancer contexts. Peripheral blood iNKT cell frequency varies between 0.01–1% of total T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells; ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Target Information

T cell receptors (TCRs) discriminate foreign versus self-peptides presented by MHC class II molecules—critical for adaptive immunity.
Self-peptide recognition is linked to autoimmune disease; mutant peptides are associated with tumors.
Superantigen engagement can cause toxic shock syndrome.
Autoantibodies to TCR Vβ segments have been found in RA and SLE, potentially modulating TH1-mediated inflammation.
Most human T cells express TCR αβ with CD4 or CD8 (SP), while a minority are double negative (DN). Increased αβ DN T cells are observed in autoimmune and immunodeficiency disorders.
γδ T cells, mainly in epithelial tissues, exhibit limited diversity and unique antigen recognition, contributing to antitumor and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.