Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 마커 연구에 적합. 다양한 종(인간, 마우스, 랫 등)에 반응하며 IHC, ICC, Flow 등 여러 응용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 성능 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | - | 199 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5–5 µg/mL | 17 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 11 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | 85 publications |
| Flow Cytometry (Flow) | - | 93 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| Functional Assay | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Dog, Ferret, Human, Mouse, Mustelid, Pig, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865119 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- Alexa Fluor Plus 594
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, M) and absent in resting cells (G0). It localizes to the nucleus during interphase and redistributes to chromosomes during mitosis. Ki-67 serves as a marker for determining cell growth fraction and tumor prognosis through the Ki-67 labeling index.
SolA15 also recognizes human, non-human primate, and canine Ki-67.
Applications reported include immunohistochemical staining of FFPE tissues, frozen sections, and immunocytochemistry.
Tested by IHC on formalin-fixed paraffin-embedded mouse skin and ICC on fixed/permeabilized C2C12 cells (≤5 µg/mL). Optimal titration recommended.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases, associated with euchromatin and heterochromatin regions. Its expression pattern changes dynamically through the cell cycle, making it a reliable proliferation marker absent in G0 cells. Ki-67 antibodies are valuable in determining tumor growth fraction and have strong correlation with tumor grade and prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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