Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio39D (39D, 3-9D)), eBioscience
T-bet 단백질을 인식하는 Mouse IgG1 단클론 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합하며, Th1 세포 발달 연구에 유용합니다. 액상 형태로 0.5 mg/mL 농도, PBS 버퍼 및 sodium azide 포함.
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Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio39D (39D, 3-9D)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio39D (39D, 3-9D) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763638 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The eBio39D monoclonal antibody reacts with mouse, rat, and human T-bet.
T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage.
T-bet deficient mice show impaired Th1 cell development, while ectopic expression of T-bet skews development toward the Th1 lineage.
T-bet expression is induced by IFN-gamma and regulates IFN-gamma expression through DNA accessibility and histone remodeling.
It also regulates IL-12Rβ and IL-2 expression and plays a role in class-switch recombination in B cells.
Applications Reported: Immunoprecipitation and immunoblotting (WB).
Applications Tested: Western blot analysis of Th1-polarized mouse CD4+ T cells (recommended starting dilution: 2 µg/mL).
Target Information
T-bet (TBX21) is a T-cell specific transcription factor with a single T-box DNA-binding domain.
It controls the expression of the Th1 cytokine interferon-gamma and is crucial in immune regulation and autoimmune disease development.
T-bet initiates Th1 lineage development from naive Th precursor cells and directs differentiation toward Th1 versus Th17.
It cooperates with Stat4 in programming chromatin architecture for Th1 gene expression.
Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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