Thermo Fisher Scientific CD44v5 Monoclonal Antibody (VFF-8)
인간 CD44v5 항원을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC(F/P), Flow Cytometry에 적합합니다. 비결합형 액상 형태로 제공되며, 4°C 또는 -20°C에 보관 가능합니다. 세포 접착 및 이동 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10 | - |
| Flow Cytometry (Flow) | 1:10 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VFF-8 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538441 |
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for staining of paraffin sections.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
CD44 cell surface antigen is a 100 kDa glycoprotein widely expressed on human leukocytes, white matter of the brain, and certain epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration.
It also plays roles in lymphocyte activation, recirculation, and homing.
CD44 expression may be upregulated in some carcinomas, potentially associated with metastatic potential.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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