Thermo Fisher Scientific Phospho-GRB10 (Tyr67) Polyclonal Antibody
GRB10 단백질의 Tyr67 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피 정제, PBS 버퍼에 0.05% sodium azide 포함. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human GRB10 around the phosphorylation site of Tyrosine 67 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553747 |
Product Specific Information
This antibody detects endogenous GRB10 protein at a molecular weight of approximately 67 kDa.
Purity is greater than 95% by SDS-PAGE.
Target Information
GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP, and a proline-rich N terminus.
A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7.
GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment but appears to bind poorly to the EGF receptor.
GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor. Similarly, GRB7 maps to the same chromosome near the EGF receptor-related protein HER2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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