Thermo Fisher Scientific Phospho-Ephrin B1 (Tyr317) Polyclonal Antibody
Phospho-Ephrin B1 (Tyr317) 폴리클로날 항체로, 인간, 생쥐, 랫트 시료에서 사용 가능. Western blot에 적합하며, 고순도(>95%) 항원 친화 크로마토그래피 정제. Ephrin-B1 신호전달 연구용. 연구용으로만 사용.
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Applications and Tested Dilution
- Western Blot (WB): 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Ephrin-B1 around the phosphorylation site of Tyrosine 317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553743 |
Product Specific Information
- Detects endogenous protein at molecular weights of approximately 38 and 45 kDa.
- Purity: >95% by SDS-PAGE.
Target Information
Ephrin-B1 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. It binds promiscuously to Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling between neighboring cells.
- Forward signaling: downstream of the receptor
- Reverse signaling: downstream of the ephrin ligand
- Known receptor interactions: EPHA4, EPHA3, EPHB4
- Biological roles:
- Central role in heart morphogenesis and angiogenesis
- Regulation of cell adhesion and migration
- Controls cellular repulsion and segregation from EFNB2-expressing cells
- May constrain orientation of longitudinally projecting axons
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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