Thermo Fisher Scientific Phospho-Ephrin B1 (Tyr317) Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:1,000

Product Specifications

Product Specific Information

• Detects endogenous protein at molecular weights of approximately 38 and 45 kDa.
• Purity: >95% by SDS-PAGE.

Target Information

Ephrin-B1 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. It binds promiscuously to Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling between neighboring cells.

• Forward signaling: downstream of the receptor
• Reverse signaling: downstream of the ephrin ligand
• Known receptor interactions: EPHA4, EPHA3, EPHB4
• Biological roles:
  • Central role in heart morphogenesis and angiogenesis
  • Regulation of cell adhesion and migration
  • Controls cellular repulsion and segregation from EFNB2-expressing cells
  • May constrain orientation of longitudinally projecting axons

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.