Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI5C1), TrueMAB

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine.
BrdU is widely used to detect proliferating cells in living tissues by incorporation into newly synthesized DNA during the S phase of the cell cycle.
Antibodies specific for BrdU enable visualization of actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved through acid or heat treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.