Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI5C1), TrueMAB
BrdU 검출용 Mouse Monoclonal Antibody로 세포 증식 연구에 적합. IHC 및 ICC/IF에 사용 가능하며, 고순도 Affinity chromatography로 정제. Lyophilized 형태로 안정적이며, 재구성 후 1 mg/mL 농도로 사용 가능.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C1 |
| Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine.
BrdU is widely used to detect proliferating cells in living tissues by incorporation into newly synthesized DNA during the S phase of the cell cycle.
Antibodies specific for BrdU enable visualization of actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved through acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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