Thermo Fisher Scientific NANOS3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (IHC)

2.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide near the carboxy terminus of the human Nanos3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NANOS3,uniProtId:P60323-1,ncbiNodeId:9606,antigenRange:173,antigenLength:173,antigenImageFileName:PA5-20537_NANOS3_P60323-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20537_NANOS3_P60323-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157855

Product Specific Information

A suggested positive control is human brain tissue lysate.

PA5-20537 can be used with blocking peptide PEP-0657.

Target Information

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2- primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3- PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3- background. Nanos3 expression can not rescue Nanos2- defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.