Thermo Fisher Scientific WNT10A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (IHC)

20 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300-350 of Wnt10a. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WNT10A,uniProtId:Q9GZT5-1,ncbiNodeId:9606,antigenRange:417,antigenLength:417,antigenImageFileName:PA5-20542_WNT10A_Q9GZT5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20542_WNT10A_Q9GZT5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152191

Product Specific Information

A suggested positive control is RAW264.7 cell lysate.

PA5-20542 can be used with blocking peptide PEP-0662.

This Wnt10a antibody is predicted to not cross-react with Wnt10b.

Target Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt`s lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.