Thermo Fisher Scientific Ly-6B.2 Monoclonal Antibody (7/4)
Rat 모노클로날 항체로 Mouse Ly-6B.2 항원을 인식합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능합니다. Protein G로 정제된 액상 형태로 제공되며, 4°C에서 보관합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:50–1:100 | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7/4 |
| Immunogen | Cultured bone marrow cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538046 |
Available Conjugated Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Rat anti-Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen.
Target Information
Ly-6B.2 is a 25–30 kDa, GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes, and some activated macrophages.
High expression levels are observed in bone marrow, spleen, lung, and lymph nodes.
N-glycanase treatment of thioglycollate-elicited peritoneal neutrophil lysates reduces the apparent molecular weight of Ly-6B.2 to approximately 15 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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