Thermo Fisher Scientific IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience
마우스 IFN-γ를 인식하는 중화용 단클론 항체로, 세포 활성화 및 사이토카인 중화 실험에 적합합니다. 낮은 엔도톡신 수준과 높은 순도로 제조되었으며, LAL 및 생물활성 시험으로 검증되었습니다. 4°C 보관, 액상 형태로 제공됩니다.
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- 16-7312-xxx (5개 옵션)
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카탈로그
5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience
Applications and Publications
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | – | 3 publications |
| ELISA | – | 6 publications |
| Neutralization (Neu) | Assay-Dependent | 2 publications |
| Functional Assay | Assay-Dependent | 2 publications |
| Inhibition Assays (IA) | – | 1 publication |
| T-Cell Activation (TCA) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R4-6A2 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866273 |
Additional Formats
Product Specific Information
Description:
The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-γ), a homodimeric 15–20 kDa cytokine secreted by Th1 cells. This antibody functions as a neutralizing antibody. IFN-γ is produced by activated T, B, and NK cells and exhibits antiviral and antiparasitic activity. It synergizes with TNF-α to inhibit proliferation of normal and transformed cells and modulates immune responses across various cell types expressing high-affinity receptors for IFN-γ. Glycosylation does not affect its biological activity.
Applications Reported: Cytokine neutralization
Applications Tested: Verified by LAL assay for low endotoxin levels and tested for IFN-γ bioactivity neutralization.
At 15 ng/mL, the antibody inhibits 50% of the biological effects of 1 ng/mL mouse IFN-γ (ND50) in an EMCV assay of L929 cell protection.
Detailed protocols on cytokine bioassays and neutralization can be found in the BestProtocols® Guide.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced mainly by T-lymphocytes and NK cells in response to antigens and cytokines.
It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits. On SDS-PAGE, it appears as 25, 20, and 15.5 kDa bands due to differential glycosylation.
The biological activity of IFN-γ is species-specific; human IFN-γ does not cross-react with mouse IFN-γ.
Functions include:
- Antiviral and antitumor activity
- Induction of MHC class I and II
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
Activation occurs via IFN-γ receptor I and II, triggering the JAK-STAT pathway.
Human IFN-γ shares ~40% sequence homology with mouse IFN-γ.
Upregulated by IL-2, FGF basic, and EGF; downregulated by vitamin D3 or DMN.
Mutations in the IFN-γ gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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